Prevalence of Escherichia Coli on Money

PREVALENCE OF ESCHERICHIA COLI ON PHILIPPINE PESO BILL WITHIN THE VICINITY OF ILOILO CITY 2012-2013 A enquiry Paper Presented to The susceptibility College of medical exam Laboratory comprehension De dismantlement Central Filipino University Iloilo city In Partial fulfilment of the Requirement for the Research 1 JUSTINE GRACE G. JIMENEA ISAAC JAMES T. LEDESMA MAJA HANNAH LOU L. LOJA ROBERTO R. MANALO JR. JIRAH L. PEDROSA MARCH 2013 CHAPTER 1 inception Background of the work A classic char momenteristic of human parasitic and b conducteriuml agents is the evolution of routes for transmittance pathogens to susceptible armaments.The env atomic number 26ment plays a critical social function in transmission to world, with umteen environmental materials attentioning as vehicles. microbic contaminants whitethorn be communicable directly, through materialise-to- fall out achieve, or indirectly, via aliment for thought or new(prenominal) breathless objects like fomites. (Rote, et al. , 2010) According to Umeh, et al (2007), taint of objects by morbific smallorganism is such(prenominal)(prenominal) a national health concern as foul materials stub be sources of transmitting pathogens. Items that solve from hand to hand argon likely to be colly with ailment causing microorganisms e limitedly if handled with ill-gotten hands or kept in dirty surroundings.Ahmed, et al (2010) denominateed out that account funds is wide interchange for corkings and run in nearly countries worldwide. Paper currencies atomic number 18 astray utilise and each up-to-dateness is exchanged many times during the time it circulates. If nearly of these written report m acey atomic number 18 soil with morbific b seconderium, there is a potential to spread these microorganism. Pope, et al (2002) mentioned, since bacterium, have been shown to spread from person to person via dawn with fomites topic money, therefore, presents a particular encounter of p ublic health.Paper currency is ordinarily and routinely passed among individuals. Thus bacterium could be spread on the go up of musical composition currency. Paper currency, as asserted by Oyero, et al (2007) stomach be pollute by droplets during spit uping, sneezing, touching with previously contaminated hands or other materials and placement on dirty surface. Paper currency is unwashedly handled by various categories of pack during transaction. The supposition that paper currency might act as environmental vehicles for the transmission of potential pathogenic microorganisms was suggested in 1970s.Various pathogens colligate with throat infection, pneumonia, tonsillitis, peptic ulcers, urino-genital tract infection, gastroenteritis and lung abscess had been reported. (Pope, et al. , 2002) The money which may get contaminated during production, storage, after production and during go for are al routes in circulation. Numerous research on currency in several countries indicated bacteriuml befoulment. A memorize in Bangladesh reported thatEscherichia coli (58%),Klebsiella(50%), staph aureus(25%), Salmonella (15%), vitamin B complex (9%), Pseudomonas (7%) and vibrio cholera (5%) were recovered from Bangladesh Paper Currency Notes (Taka). Ahmed, et al,. 2010) In a nonher hold carried out in Nigeria, the bacteria stranded E. coli (80%), Aerobacter (59%), Salmonella (40. 9%), yeast cellular telephones (36. 4%), Streptococcus faecalis(31. 8%), staphylococcus aureus( 27. 3%) and the coagulase shun staphylococci (18. 2%). (Umeh, et al. , 2007) A similar make was as well as conducted in Nepal and reported thatstaphylococciaureus, S. Epidermidis, Streptococcus pyogenes, Klebsiella pneumonia, Salmonella choleraesius, E. coli, Enterobacteraerogenesand cloaceaewere spaced from paper money of Nepal. (Lamichhane, et al. 2009) In a nonher study in India, carbon% of the currency nones of Indian upee were contaminated with bacteria. Mainly cardi nal species including E. coli, Proteus spp. And Staphylococcus aureuswere unaffectionate. (Bhat, et al. , 2010) In a nonher study in India, currency warnings of contrary denominations from different occupational multitudes were evaluated for isolation of microbial contaminants and Staphylococcus aureu, E. coli, Bacillus spp. , Klebsiella spp. , Proteus mirablisand fungus like Aspergillusnigerand Fusariumwere isolated from these paper currency notes. (Rote, et al. 2010) Although, a lot of studies on the microbiological status of paper currency have been carried out elsewhere, data on the microbial befoulment of the peso ( Philippine currency) is s stick outty. In order to know the prevalence of Escherichia coli on Philippine Peso annotation indoors the vicinity of Iloilo City in the year 2012-2013, this study is to be conducted. normal Objectives What is the prevalence rate of E. coli befoulment on the Philippine peso street arab of Public Utility Jeepney (PUJ)? particular proposition Objectives 1. ) What is the prevalence rate of E. coli contamination on the 20 peso schnozzle? . ) What is the prevalence rate of E. coli contamination on the 50 peso bill? 3. ) What is the prevalence rate of E. coli contamination on the 100 peso bill? Research Paradigm In babelike variableDependent variable LocationPublic Utility Jeepney (PUJ)Value20 PHP50 PHP100 PHP prevalence rate of Escherichia coli contamination Figure 1. Schematic Diagram of the Study Definition of Terms To provide uncloudedness and understanding, the following terms were defined conceptually and operationally. bacterium- are minute, unicellular organisms that re name by binary fission.They are considered prokaryotes due to the absence of nuclear membrane. They possess both DNA and RNA. They sensitive to penicillins, tetracycline and chloramphenicol. (Moraleta, Review of Microbiology) In the study, the bacteria is the E. coli to be identified. Escherichia coli- or E. coli is a bacterium th at is comm only rig in the gut of humans and other warm-blooded animals. While well-nigh strains are harmless, whatsoever support establish severe solid foodborne ailment. E. coliinfection is usually transmitted through consumption of contaminated piss or food, such as undercooked warmness products and raw draw.Symptoms of disease include abdominal cramps and diarrhoea, which may be bloody. Fever and vomiting may alike turn over. Most patients recover inwardly 10 days, although in a few cases the disease may locomote life-threatening. (WHO, 2012) As subprogramd in this study,E. coli is the subject of the study and it is isolated in the surfaces different peso bill. befoulment-The act or process ofcontaminating pollution defilement taint also, that which contaminates. (http//www. merriam-webster. com/) In this study, contamination refers how high or low E. oli bacteria direct is erect on the surface of the peso bill. Denomination-a value or surface of a series of values or sizes. (http//www. merriam-webster. com/) As utilise in thisstudy, 20, 50, 100 are use as denomination. Paper bill- ( practically known as abill,paper moneyor simply anote) is a type ofnegotiable instrumentknown as apromissory note, made by abank, payable to the bearer on demand. (http//en. wikipedia. org) In this study, paper bill was tested for the charge or absence of E. coli. Philippine peso- is the currency of thePhilippines.The peso is usually denoted by the symbol ? . Other ways of writing the Philippine peso sign are PHP, PhP, Php, and/or P. (http//www. wikipedia. org/) As used in this study, Philippine peso paper bill was tested for the presence of E. coli. Public Utility Jeepney (PUJ)- are the most popular representation ofpublic transportationin the Philippines. (http//www. wikipedia. org/) In this study, this is the location of the Philippine peso billwhere the E. coli is to be isolated. Signifi bay windowce of the Study The result of the study will benefit the followingDepartment of Health. may provide in shapeation on how to properly handle and care for money to prevent the risk of Escherichia coli contamination from hand-to-hand communication. The Medical Researchers. They may use this study as reference for future studies oddly in the prevention of the deadly disease fixd by Escherichia coli on the circulating money. Commercial Banks. They may have an idea that Escherichia coli can inhabit the paper bill and commence such disease. Hence, they will come up bacteria-free money. Jeepney Drivers.May help them in nurseing their selves from world the courier of contaminated money from one person to another. The public. The result will provide in variantation and ken about money contaminated by Escherichia coli circulates, and find ways in preventing disease and reduce mortality. forthcoming tec. The result of the study may also be used by other future researcher as a reference or basis for future researcher or investigation. Scop e and demarcation of the Study This study aims to furbish up the prevalence of Escherichia coli contamination on Philippine peso bill inwardly the vicinity of Iloilo City.A total of 45 paper currency of denominations, fifteen paper currencies of each 20 PhP, 50 PhP and 100 PhP in circulation were stochasticly peaceful in 15 jeepneys at different times. Coins were excluded from the study. Paper currencies were gathered from Jaro-CPU Public Utility Jeepney (PUJ) from CPU to Iloilo City route and vice versa. This study is conducted during the month of March until May in the year 2013. Persons discussion the bill were asked to deposit them in sterile polyethylene bags, sealed and interpreted to the Medical Laboratory Science Research Laboratory of Central Philippine University, for analysis. CHAPTER 2REVIEW OF RELATED LITERATURE AND STUDIES This chapter deals with the related literature and studies relevant to the study of Escherichia coli contamination on paper money bills. Money in the form of notes or coins is handled by e very(prenominal)one, and dirty money (money contaminated with pathogenic microorganisms) is always in circulation. pollution may occur during production, during storage after production, and during use. Microorganisms on the skin can be transferred from cashiers, salespeople and the planetary public to the currency notes that they handle. Contamination from the anal region, wounds, nasal secretions and aerosols enerated by sneezing and coughing are potential sources of transfer of microorganisms to currency notes during discourse. Staphylococcus epidermidis, Pseudomonas aeruginosaandKlebsiellaaerogeneshave been reported to survive well on the skin,and are known to be transferred from fabrics to hand as well as from hand to fabrics. (Tswana, 2000) Items that are passed from hand to hand are likely to be contaminated with disease-causing bacteria particularly if carried with unclear hands, or kept in a dirty environment. Similarly, pa per currency is widely exchanged for goods and services in countries worldwide.Currency contamination with pathogenic bacteria is of much public health concern as contaminated materials can be sources of serious pathogens. Paper currency, therefore presents a particular risk to public health, since they go in circulation and contaminate the hands of others transmitting pathogenic organisms in the process. (Uneke&Ogbu, 2007) Pathogenic bacteria that may survive on the currency banknotes may serve as a potential source of enteropathogens causing food poisoning because food vendors handle and serve food and at the same time handle currency banknotes as they sell. Cardoenet al. , 2009 Lamichhaneet al. , 2009) The incidence of food poisoning is on the extend worldwide, although it is estimated that only 10% of cases are reported just the tip of the iceberg. Money bill play a major role in transmission of pathogenic bacteria. Some mathematical models have been developed to help understa nd the movements of currency and how this might contribute to the global spread of disease. One of the main sources of pathogenic or food-poisoning bacteria is people. People commonly carry these bacteria in their nose, mouth, wounds and intestines, and on their skin.There are several reports of the particular of microorganisms, in particular bacteria, on cash banknotes and coins. A study in the US showed that only 6% of banknotes tested were free from microbial contamination. Dirty money Modern banknotes are made from a special blend of 75% cotton and 25% linen with small segments of fibre, so paper money is something of a misnomer. The paper is referred to as the substrate during the manufacturing process this is an appropriate name as bacteria require a substrate for sprainth.The cotton/linen/fibre combinations of banknotes produce a strong bond and do not pull apart, unlike the fibres of ordinary paper. The reasonable life span of a low denomination paper banknote is about 2 4 months. In the early 1980s, an American Bank developed polyethylene fibres for use as currency and they are still in use in some Central American countries. Non-fibrous, non-porous, polymer banknotes, developed by the Reserve Bank of Australia, were first issued as currency in 1988. The banknotes were made from biaxially orient polypropylene that made them more(prenominal) durable, with security features that rendered them difficult to counterfeit. Aidoo, 2011) Various routes are known that lead to the contamination of paper bill. Handling of it results in the transfer of bacteria from money onto hands or from hands onto money. Individuals who cough or sneeze into their hands can easily transfer bacteria onto currency when they handle it. Viruses may also be transmitted when give people touch surfaces, such as banknotes, that are tallyly touched by others. In the hospitality and catering industry, it is unacceptable for staff to use one hand to handle food and the other to ha ndle money. regimen, any cooked or uncooked, may conduct bacteria which can be transferred either directly or indirectly through a medium such as a work surface onto currency. This may occur particularly with street food, mobile food vendors and in retail outlets at the counter. Obviously, the transfer of pathogenic bacteria to food that is ready to be eaten and that requires no advertise heat treatment could have serious consequences. Should money be handled amid hand washing and food discussion, then it is equally important to repeat the process of hand washing before handling risky food.Wallets, purses, cash registers and other closed environments are conducive for microbial growth because they create warm and wet conditions. Transfer of micro-organisms from one banknote to another in such closed environments may also be common. (Aidoo, 2011) Money on which pathogenic microorganisms might survive represents an often overlooked reservoir for enteric disease (Michaels, 2002) . In most parts of the developed world, there is a popular belief that the simultaneous handling of food and money contributes to the incidence of food-related public health incidents (Food Science Australia FSA, 2000).It goes without saying that bacteria are everywhere in the environment and most of these microbes are harmless to humans. Should germs on money worry us? Surprisingly, studies of bacteria on money are scarce. In 1972 a study in the Journal of the American Medical Association studied bacteria from 200 coins and bills and found harmful germs like fecal bacteria and Staphylococcus aureus on 13 percent of coins and 42 percent of notes. The study concluded Money is truly dirty. (http//christophreilly. hubpages. com/) Micro-organisms commonly associated with banknotes include Staphylococcus aureus, ? haemolytic Streptococcus, Enterobacter spp. , Acinetobacter spp. , Pseudomonas spp. , Bacillusspp. , Escherichia coli, Salmonella spp. , viruses, yeasts and moulds. Some of t hese bacteria are pathogenic, maculation others may cause opportunistic infections. The predominant, recurrent pathogenic bacteria found on banknotes are S. aureus, Bacillus spp. and Escherichia spp. Many members of the family Enterobacteriaceae are found in the gut of animals and humans, and their presence in food or on inanimate objects and surfaces is a good indicator of poor hygiene.Members of the genera Enterobacter, Escherichia and Klebsiella isolated from banknotes may not themselves cause serious illness however, their isolation from money may indicate the presence of other pathogenic organisms. Escherichia coliis an important member of the fecal coliform group and its presence on banknotes is of public health concern, peculiarly as some strains can of rail line cause serious illness. Species of the Gram-negative genus Pseudomonas, which can cause serious opportunistic infections, have also been isolated from banknotes.S. aureus is the predominant bacterium present on the surface of banknotes and it is also a common cause of food poisoning. This organism is indicative of poor measures of hygiene particularly during food handling and/or preparation. Many people in the adult population carry S. aureus on their skin, in their nasal cavity, and in septic cuts, boils and spots. Coagulase-positive S. aureus is quick isolated from banknotes and the toxins it produces may cause toxic shock syndrome. It has also been implicated in pneumonia.Streptococci are part of the normal microflora in the mouth, skin, intestine and upper respiratory tract of humans. Although many streptococcal species are non-pathogenic, some have been implicated in meningitis and pneumonia. Bacillus spp. are spore-formers and can withstand harsh, adverse conditions, such as drying. Some species, for example Bacillus cereus, cause dickens types of food poisoning diarrhoeal (heat-labile toxin) and emetic (heat-stable toxin). (Aidoo, 2011) The surface of paper banknotes is not smooth, b ut irregular, and can harbour many different types of microorganisms.The two main factors that determine the occurrence of bacteria on currency are (i) the material that the banknotes are made from and (ii) the age of the banknote. bacterium have enormous capabilities to allow them to survive in adverse conditions. Two of the most important strategies for natural selection are their efficiency to adhere to surfaces and the tycoon to form biofilms (multicellular aggregates). Members of some genera, such as Bacillus, may form spores and can survive attached to banknotes for many years. Formation of a biofilm or a spore is controlled by contagious activity of the bacterium.Bacterial cells on banknotes are measured by the number of closure-forming units (c. f. u. ) per cm2 of banknote. A banknote may post up to 106c. f. u. cm2, whilst a coin may have up to 103c. f. u. cm2. Studies have shown that polymer-based banknotes often have a relatively low bacterial count compared with th e cotton-based paper banknotes. This may be due to various physicochemical parameters of polymers. For example, a negatively charged and hydrophilic synthetic polymer would adversely affect bacterial attachment. (Vriesekoop et. al. , 2010) Escherichia coli, also known as E. oli is a Gram-negative, rod-shaped bacterium that is commonly found in the get intestine of warm-blooded organisms (endotherms). Most E. coli strains are harmless, but some types can cause serious food poisoning in humans, and are occasionally trustworthy for food contamination. (CDC National Center for Emerging and zoonotic Infectious Diseases, 2012) The harmless strains are part of the normal flora of the gut, and can benefit their hosts by producing vitamin K2, and by preventing the establishment of pathogenic bacteria inside the intestine. (Hudault et al. , 2001).Fecaloral transmission is the major route through which pathogenic strains of the bacterium cause disease. Cells are able to survive impertinent the body for a limited inwardness of time, which makes them ideal indicator organisms to test environmental samples for fecal contamination. There is, however, a growing body of research that has examined environmentally persistent E. coli which can survive for extended periods outside of the host. (Ishii &Sadowsky, 2008) Escherichia coli (or E. coli) is the most prevalent infecting organism in the family of gram-negative bacteria known as enterobacteriaceae.E. coli is often referred to as the best or most-studied free-living organism. more(prenominal) than 700 serotypes of E. coli have been identified. The O and H antigens on the bacteria and their flagella distinguish the different serotypes. Indeed, some E. coli are beneficial, while some cause infections other than gastro intestinal infections, such as urinary tract infections. The E. coli that are responsible for the numerous reports of contaminated foods and beverages are those that produce Shiga toxin, so called because the toxin is virtually identical to that produced by Shigelladysenteria type 1. Eisenstein et al, 2000) Strains of E. coli that causes dissipation in man Enteropathogenic E. coli (EPEC) causes diarrhea, but the molecular mechanisms of colonization and aetiology are different. EPEC lack fimbriae, ST and LT toxins, but they use an adhesin known as intimin to bind host intestinal cells. This virotype has an array of virulence factors that are similar to those found in Shigella, and may possess a shiga toxin. Adherence to the intestinal mucosa causes a rearrangement of actin in the host cell, causing crucial deformation. EPEC cells are moderately invasive (i. . they enter host cells) and elicit an inflammatory response. A change in intestinal cell ultrastructure due to attachment and effacement is likely the prime cause of diarrhea in those afflicted with EPEC. (University of WisconsinMadison Department of Bacteriology, 2007) Enterotoxigenic E. coli (ETEC) Enterotoxigenic Escherichia coli, or ETEC, is the name given to a group of E. coli that produce special toxins which stimulate the lining of the intestines causing them to secrete excessive fluid, thus producing diarrhea. (cdc. gov, 2004) Enteroinvasive E. oli (EIEC) this infection causes a syndrome that is identical to Shigellosis, with profuse diarrhea and high fever. EIEC are exceedingly invasive, and they utilize adhesin proteins to bind to and enter intestinal cells. They produce no toxins, but severely damage the intestinal wall through mechanical cell destruction. ( Lan et al, 2004). Enteroaggregative E. coli (EAEC) is a subgroup of diarrhoeagenic E. coli (DEC) that during the past decade has received increasing attention as a cause of watery diarrhea, which is often persistent. EAEC have been isolated from children and adults worldwide.As well as sporadic cases, extravasations of EAEC-caused diarrhea have been described. (sgmjournals. org, 2003) diffusely adherent E. coli (DAEC) may cause dise ase in immunologically naive or malnourished children. Discrepancies among epidemiological studies could be explained by age-dependent susceptibility to diarrhea or by the use of an inappropriate detection method such as DNA probing. The current prospective case-control study was done to determine the role of DAEC strains as a cause of acute diarrhea in northeastern Brazil, where childhood diarrhea is endemic. ( M. M. Levine. 1990) Shiga toxin-producing E. oli (STEC) Some strains of E. coli produce a toxin called Shiga toxin that causes diarrhea and can lead to severe illness. These Shiga toxin-producing E. coli are sometimes called STEC (pronounced S-TECK). STEC can be spread from oxen and other animals to people through raw or undercooked meat, unpasteurized milk, or through mop up with an septic animal. People can also get infected by consuming contaminated water, raw produce or unpasteurized juice or cider. Foods can become contaminated with bacteria from manure in the field or from raw beef or raw beef juices in the kitchen.People with STEC who do not wash hands well after a bowel movement can spread it to others. (kingcounty. gov, 2005) The genera Escherichia and Salmonella diverged nearly 102 million years ago, which coincides with the divergence of their hosts the former being found in mammals and the latter in birds and reptiles. This was followed by a split of the escherichian ancestor into five species (E. albertii, E. coli, E. fergusonii, E. hermannii and E. vulneris. ) The last E. coli ancestor split between 20 and 30 million years ago. ( Lecointre et al. 2007) In 1885, a German pediatrician, Theodor Escherich, first discovered this species isolated from the feces of newborns and called it bacterium coli commune due to the fact it is found in the colon and early classifications of Prokaryotes fit(p) these in a handful of genera based on their shape and motility (at that time Ernst Haeckels classification of Bacteria in the commonwealth Mone ra was in place). bacteria coli was the type species of the now invalid genus Bacterium when it was revealed that the former type species (Bacterium triloculare) was missing.Following a revision of Bacteria it was reclassified as Bacillus coli by Migula in 1895 and later reclassified in the pertly created genus Escherichia, named after its original discoverer. ( Castellani and Chalmers, 2009) The genus belongs in a group of bacteria informally known as coliforms, and is a member of the Enterobacteriaceae family (the enterics) of the Gammaproteobacteria. (Garrity, 2005) Escherichia coli encompass an enormous population of bacteria that exhibit a very high degree of both genetic and phenotypic diversity. Genome sequencing of a self-aggrandising number of isolates of E. oli and related bacteria shows that a taxonomic reclassification would be desirable. However, this has not been done, largely due to its medical importance and E. coli remains one of the most diverse bacterial species only 20% of the genome is common to all strains. (Lukjancenko et. al. , 2010) In fact, from the evolutionary point of view, the members of genus Shigella (S. dysenteriae, S. flexneri, S. boydii, S. sonnei) should be classified as E. coli strains, a phenomenon termed taxa in disguise. Similarly, other strains of E. coli (e. g. he K-12 strain commonly used in recombinant DNA work) are sufficiently different that they would merit reclassification. (Lan Reeves, 2002) A strain is a sub-group within the species that has unique characteristics that distinguish it from other strains. These differences are often detectable only at the molecular level however, they may result in changes to the physiology or lifecycle of the bacterium. For example, a strain may gain pathogenic capacity, the ability to use a unique carbon source, the ability to take upon a particularecological niche or the ability to resist antimicrobial agents. Different strains of E. oli are often host-specific, making it po ssible to determine the source of fecal contamination in environmental samples. For example, knowing which E. coli strains are present in a water sample allows researchers to make assumptions about whether the contamination originated from a human, another mammal or a bird. (Feng et. al. , 2002) The bacteria normally adhere to the mucus or the epithelium on the wall of the intestines, and a single strain can last for months or years. E. coli is one of the most studied and best understood organism, but the organisms role and how it colonizes isnt very well understood.Freters Nutrient-Niche theory best describes E. colis role The ecological functions of E. coli depend on the nutrient availability within the intestines of the host organism. Since there are so many different nutrients that can be found within the intestines, it is said that they contain a balanced ecosystem because there are so many different nutrient-defined functions where the E. coli colonize. This is dependent on th e preferred nutrient for that particular population occupying the niche. E. coli can also be found outside of the body in faecally-contaminated environments such as water or mud. (bioweb. wlax. edu, 2008) The optimal growth of E. coli occurs at 37 degrees Celsius but some laboratory strains can multiply at temperatures of up to 49 degrees C. Growth can be driven by aerobic or anaerobic respiration. (answers. ask. com, 2010) As E. coli is part of the common micro? ora in the large intestine, it is accustomed to a pH of 7-8. As glucose is absorbed in the small intestine, the E. coli would be used to low concentrations. However, as glucose is its energy source, if excess glucose were available for consumption, it would be expected that the E. coli would utilise it and grow at a faster rate.Salt (NaCl) is absorbed in the colon, so the amount of salt that the E. coli is exposed to depends on how much salt is consumed by the host organism. However, because of the mechanism of osmosis, ext remely high levels as well as complete absence of salt could be lethal to E. coli bacteria. (APUA, 2007 How Antibiotics Work the Mechanism of Action , Alliance for the circumspect Use of Antibiotics,) You get an E. coli infection by coming into click with the feces of humans or animals. This can happen when you drink water or eat food that has been contaminated by feces. Healthwise, 2011) E. coli infection can cause human illness when E. coli is ingested through various modes of transmission, including through food and water sources, animal-to-human connexion, and person-to-person contact. (Clark, 2012) And according to the (Centers for Disease Control and Prevention) CDC, the transmission of these bacteria to humans may occur in the following room Meat, such as beef from cows, may become contaminated when organisms are accidently goed in with beef, especially when it is ground. Meat contaminated with E. coli does not smell and looks normal.It is important to thoroughly cook t he beef. Infection may occur after swimming in or drinking water that has been contaminated with E. coli. Person-to-person contact in families and in child-care and other institutional-care centers are also places where the transmission of the bacteria can occur. However, the CDC also indicates the way E. coli is transmitted may change over time. (medicalcenter. osu. edu, 2006) To help avoid food poisoning and prevent infection, handle food safely. Cook meat well, wash fruits and vegetables before eating or cooking them, and avoid unpasteurized milk and juices.You can also get the infection by swallowing water in a swimming pool contaminated with human waste. (nlm. nih. gov, 2012) Pathogenic Escherichia coli, or E. coli, is believed to mostly live in the intestines of cattle. Certain serotypes of E. coli have also been found in the intestines of chickens, sheep, and pigs. (Clark, 2012) E. coli in food E. coli can get into meat during processing. If the infected meat is not cooked t o 160F (71C), the bacteria can survive and infect you when you eat the meat. This is the most common way people become infected with E. coli.Any food that has been in contact with raw meat can also become infected. Other foods that can be infected with E. coli include Raw milk or dairy farm products. Bacteria can spread from a cows udders to its milk. Check the labels on dairy products to make sure they contain the word pasteurized. This means the food has been heated to destroy bacteria. Raw fruits and vegetables, such as lettuce, alfalfa sprouts, or unpasteurized apple cider or other unpasteurized juices that have come in contact with infected animal feces. E. coli in water Human or animal feces infected with E. oli sometimes get into lakes, pools, and water supplies. People can become infected when a contaminated city or town water supply has not been properly treated with chlorine or when people perchance swallow contaminated water while swimming in a lake, pool, or irrigatio n canal. E. coli from person-to-person contact The bacteria can also spread from one person to another, usually when an infected person does not wash his or her hands well after a bowel movement. E. coli can spread from an infected persons hands to other people or to objects. Person-to-person transmission of E. oli occurs through a fecal-oral route, and is particularly common among infants and young children due to their unrefined hygienic practices. Person-to-person transmission of E. coli has also been known to occur between infected individuals and their caregivers, and between infected handlers of certain objects. (WebMD, 2010) In a study entitled Microbial Contamination in 20-Peso Banknotes in Monterrey, Mexico, the banknotes came from various sources, including banks, toll booths, convenience stores, restaurants, cafeterias, and yogurt stores, among others.Samples were randomly obtained and banknotes were placed in a sterile polyethylene bag. The bag was sealed and the sample was interpreted to the laboratory. All currency banknotes were in good visible condition. Each banknote was placed in 5-mL sterile saline for 24 hours. A sterile, cotton-tipped pat was briefly introduced in the saline and the swab was seeded with a portion of the saline homogenized in blood agar plates and incubated for 48 hours at 37C in aerobic conditions. Plates were then examined for bacterial growth and the colonies underwent Gram stain.Gram-negative colonies were grown on Eosin methylene blue agar plates and identified with the crystallizing Identification System (Becton Dickinson). Gram-positive cocci were grown on azide agar plates and identified by conventional biochemical tests. Identification of yeasts was performed with CHROMagar Candida (Becton Dickinson). Of the 70 currency banknotes on which bacteriological analysis was conducted, 48 (69%) were found to be contaminated with several microbial species (Table 1).Sixteen species isolates were obtained from the bankno tes 14 bacterial species (four 23% Gram positive and 10 63% Gram negative) and two (13%) yeast species. The most prevalent species observed was Candida kruseii (19 banknotes, 27%) followed by Burkholderiacepacia (nine banknotes, 13%). Of the 70 banknotes include, 22 (31%) showed no growth. Four bills (5. 7%) yielded bacteria considered pathogenic to healthy hosts and the other 44 contaminated bills (63%) yielded bacteria considered potentially pathogenic to hospitalized or immunocompromised hosts.Additionally, 11 bills showed more than one microbial species. Pope and co-authors analyzed 68 $1 bills quiet from a school and a grocery store and found that five (7%) were contaminated with pathogenic bacteria, 59 (87%) were contaminated with opportunistic pathogens, and just four (6%) were free of bacteria (Pope et al. , 2002). Unlike the study by Pope and co-authors, in our study 31% of the banknotes were negative. Our results are similar to a previous issue (Abrams Waterman, 1972), i n which 70% of banknotes were contaminated with bacteria.Of these banknotes, 60% contained pathogens, including S. aureus, E. coli, and P. aeruginosa. Medical staff office seem to play an important role in contamination of paper currency, since it has been reported that 13% of coins and 42% of currency collected from laboratory personnel were contaminated with S. aureus, E. coli, Klebsiella sp. , and Proteus mirabilis (Abrams Waterman, 1972). Additionally, the culture of 100 banknotes and 102 coins collected from medical personnel showed that 3% of coins and 11% of banknotes were contaminated with opportunistic pathogens (KhinNwe et al. 1989). In our study, we did not include banknotes collected in any hospital, but banknotes collected near hospital facilities were contaminated with opportunistic pathogens such as B. cepacia and A. baumannii. Uneke and Ogbu assessed the potential of Nigerian currency notes to act as environmental vehicles for the transmission of pathogenic parasite s and bacteria. Currency notes obtained from four major cities in Nigeria were evaluated according to regulation techniques. Fifty-four (21. 6 percent 95 percent CI 16. 50-26. 0) of the first batch of 250 notes, which underwent parasitological analysis, were contaminated with enteric parasites 133 (53. 2 percent 95 percent CI 47. 02-59. 39) of the second batch of 250 notes, which underwent bacteriological analysis, were found to be contaminated with bacteria. leechs that were isolated from the notes included Ascarislumbricoides (8. 0 percent), Enterobiusvermicularis (6. 8 percent), Trichuristrichiura (2. 8 percent), and Taenia species (4. 0 percent). Bacteria that were isolated were Streptococcus species (21. percent), Staphylococcus species (12. 8 percent), Escherichia coli (13. 2 percent), and Bacillus species (5. 6 percent). Among dirty/mutilated currency notes, parasite contamination and bacterial contamination were both significantly (p . 05) more pervasive (30. 6 percent and 73. 8 percent, respectively) than they were among plumb and surge currency notes. Lower-denomination notes were more likely to be contaminated than were higher-denomination notes, although the difference was not statistically significant (p . 05).Parasite contamination and bacteria contamination were both most frequent in notes obtained from butchers and beggars. These results suggest that currency notes may be contaminated, especially with bacteria and enteric parasites, and may serve as sources of infection. The possibility that currency notes might act as environmental vehicles for the transmission of potential pathogenic microorganisms was suggested in the 1970s (Abrams Waterman, 1972). Paper currency is widely exchanged for goods and services in countries worldwide.It is used for every type of commerce, from buying milk at a local store to trafficking in sex and drugs. All this trade is hard on currency, with lower-denomination notes receiving the most handling because they are exchanged many times (Gadsby, 1998). Although paper currency is made to take abuse (up to 4,000 folds in each direction) in most parts of the world, including in Nigeria (where paper currency is a rugged mix of 75 percent cotton and 25 percentlinen), it lasts less than a few years in circulation (Gadsby, 1998).The average U. S. dollar, for instance-like most currency notes worldwide-lasts a mere 18 months (Gadsby). Paper currency also provides a large surface area as a breeding ground for pathogens (Podhajny, 2004). Oddly, publications regarding the degree to which paper money is contaminated with bacteria are few and far between, as the authors found when they conducted a Medline search in declination 2005 (Abrams Waterman, 1972 El-Dars Hassan, 2005 GoktasOktay, 1992 Jiang Doyle, 1999 Khin et al. 1989 Michaels, 2002 Pope et al. , 2002 Singh et al. , 2002 Xu et al. , 2005). Furthermore, the search found no documented study of the parasitological status of currency notes (as o f December 2005). scientific information on the contamination of money by microbial agents is also lacking in most create countries in sub-Saharan Africa, including Nigeria. This dearth of information may have contributed to the absence of public health policies or legislation on currency usage, handling, and circulation in many parts of Africa.Although the studies done in the unite States and Australia have had no major impact on policies or legislation on currency handling and circulation in those countries, they have fostered a higher level of public awareness about the potential for currency contamination by microorganisms (Dow Jones News, 1998 FSA, 2000 Michaels Jiang Doyle Pope et al. ). In the United States, a whole division of the Department of exchequer deals with what is termed mutilated currency, and the department Web site boasts many examples of beleaguered, burned, buried, water- change money (Siddique, 2003).Isolation of various bacterial contaminants from the curr ency notes was performed via standard techniques described previously (Gilchrist, 1993 Singh et al. , 2002). Briefly, a sterile, cotton-tipped swab moistened with sterile physiological saline was used to swab both sides of the currency note. The swabs were directly inoculated on blood agar and MacConkey agar. The pairs of inoculated media were incubated aerobically at 35-37C for 24 hours and then examined for bacterial growth according to standard protocol described previously (Cheesbrough, 2000).The authors isolated bacteria by assessing colony characteristics and Gram chemical reaction, and by conducting catalase and coagulase tests hemolysis, sugar fermentation, and other biochemical tests, including tests for indole production, citrate utilization, and urase activity triple sugar iron (TSI) agar tests (for glucose, sucrose, and lactose fermentation) gas and hydrogen sulfide production tests and oxidase tests, according to protocols described previously (Cheesbrough). Bacteria we re identified but were not quantified.Microbial contaminants can be transmitted directly by hand-to-hand contact or indirectly via food or inanimate objects like banknotes and doorknobs or other objects that come into contact with multiple human hands. Banknotes may be contaminated during storage and exchange, with lower-denomination banknotes receiving the most handling and therefore more contamination (Abrams Waterman, 1972). In general, a considerableer number of bacteria have been reported on banknotes than on coins. Banknotes are an excellent transport medium for different types of microorganisms because they are commonly passed among individuals.Thus, handling money may be a route for transmission of infections (Xu et al. , 2005). We analyzed a sample of 70 20peso bills to isolate and diagnose bacteria or yeast that may be present on the surface of these banknotes. A upstart study that included 1,280 banknotes from 10 countries, including Mexico, reported that pathogens cou ld only be isolated after enrichment and their mere presence did not appear to be alarming (Vriesekoop et al. , 2010). The authors discussed that the presence of bacteria on banknotes is influenced by the material used for the banknotes (polymer based vs. otton based) and the age of the banknotes. They stated that the average number of bacteria encountered on the polymer banknotes was approximately 25% of that found on cotton-based banknotes (Vriesekoop et al. , 2010). That study showed a lower percentage of contamination in Mexican banknotes in comparability to banknotes from China, the U. S. , the UK, and the Netherlands, among others. We analyzed only polymer-based banknotes in good condition, but the presence of contamination was detected in most banknotes analyzed without any enrichment procedure.In that study, the only contaminations detected in Mexican banknotes were E. coli, S. aureus, and a low percentage of Bacillus cereus, which were interpreted as an indicator of poor hy giene, background microorganism, and the ability of spore-forming bacteria to persist on banknotes, respectively. In our study, we detected S. aureus and Bacillus spp. but we did not detect E. coli. It is important to point out that the 10-country study included currencies obtained only from food outlets and for this reason the results from that study and ours cannot strictly be compared. Source Microbial Contamination in 20-Peso Banknotes in Monterrey, Mexico Rocha-Gamez, Judith Tejeda-Villarreal, capital of Minnesotaa Nelly, QCB Macias-Cardenas, Patricia Canizales-Oviedo, Jorge Garza-Gonzalez, Elvira et al. Journal of Environmental Health 75. 2 (Sep 2012) 20-3. ) In another study, entitled Potential for Parasite and Bacterial Transmission by Paper Currency in Nigeria, Bacteria that were isolated were Streptococcus species (21. 6 percent), Staphylococcus species (12. 8 percent), Escherichia coli (13. 2 percent), and Bacillus species (5. 6 percent).Among dirty/mutilated currency not es, parasite contamination and bacterial contamination were both significantly (p . 05) more pervasive (30. 6 percent and 73. 8 percent, respectively) than they were among clean and mint currency notes. Lower-denomination notes were more likely to be contaminated than were higher-denomination notes, although the difference was not statistically significant (p . 05). Personal hygiene to reduce risk of infection is recommended. Microbial contaminants may be transmitted either directly, through hand-to-hand contact, or indirectly, via food or other inanimate objects.These routes of transmission are of great importance in the health of many populations in developing countries, where the frequency of infection is a general indication of local hygiene and environmental sanitation levels. (Cooper, 1991). Paper currency is widely exchanged for goods and services in countries worldwide. It is used for every type of commerce, from buying milk at a local store to trafficking in sex and drugs . All this trade is hard on currency, with lower-denomination notes receiving the most handling because they are exchanged many times (Gadsby, 1998).Bacteriological analysis indicated that 133 (53. 2 percent, 95 percent CI 47. 02-57. 39) of the second batch of 250 notes were contaminated with bacteria 37 (52. 9 percent) of the 70 clean notes and 96 (73. 8 percent) of the 130 dirty/mutilated notes. uncomplete parasite ova nor bacteria were found on the mint notes. Contamination was related to denomination of currency. Contamination with bacteria was most prevalent among the N5 notes (62. 2 percent) and least prevalent among the N500 notes (32. 0 percent). Contamination was also related to the physical condition of the currency.The dirty/mutilated notes also had the highest prevalence of bacterial contamination (73. 8 percent). Among the clean notes, 52. 9 percent were contaminated with bacteria. Chi-square analysis indicated a statistically significant association between bacterial contamination and currency condition (? sup 2 = 79. 030, df = 2, p. 05). In the study reported here, a random approach was adopt for currency sampling whatever money was provided was collected. The implication is that the majority of the people are exposed to dirty notes.In Nigeria, poor-currency-handling culture is widespread, and there is indiscriminate abuse of currency notes. A great majority of the populace does not carry money in wallets, and squeezing of currency notes is a common occurrence. Women, especially among the unenlightened, often place money underneath their brassieres, while men place theirs in their socks. These activities not only enhance currency contamination but may also increase the risk of infection from contaminated notes. The situation is further compounded by the inability of the Nigerian government to consistently withdraw old, worn-out, and mutilated notes from circulation.The presence of damaged currency notes and the failure to consistently withdra w them from circulation are common phenomena in many parts of Africa and Asia (Gadsby, 1998 Podhajny, 2004). The persistence of damaged or terribly mutilated notes in active circulation (some so damaged that they can no longer be stuck together) could elevate their contributory role in transmission of some pathogens, thereby constituting potential public health hazard. ( Source Uneke, C. and Ogbu, O. Potential for parasite and bacteria transmission by paper currency in Nigeria. J Environ Health, 69 (2007) 5460. (1)) CHAPTER 3RESEARCH DESIGN AND METHODOLOGY theatrical role of the Study The purpose of the study is to determine the prevalence of E. coli contamination on Philippine Peso bill on Public Utility jeepney (PUJ), specifically on twenty (20), fifty (50), one hundred (100) pesos. Subjects of the study The subject of the study is the fifteen (15) Public Utility Jeepney drivers routing Jaro-CPU and City Proper bound. On each driver, three (3) Philippine Peso bill samples with am ounts 20, 50 and 100 are to be collected. Data Gathering 45 peso bills are to be used as sample and will be collected on the month of March 2013 at around 1000 1100 in the morning.The samples will be taken randomly from 15 Public Utility Jeepney (PUJ) of Jaro Cpu Route. In each jeepney, three(3) different values 20, 50, and 100 Php are to be taken and tested, so the total number of samples is 45. The samples collected will be placed in a sealable plastic container, so as to preserve the contaminants on the peso bill. The paper bill will be brought immediately to Central Philippine University, particularly at the Medical Laboratory Science Department Research Lab for confirmatory testing of E. coli. Preparation of Laboratory Materials Needed The following are needed to conduct our study . ) Sterile cotton swab will be used in obtaining the possible contaminant on the surface of the paper bill. b. )Eosin Methylene rich Agar will be used in the confirmatory test for the presence o f E. coli The following are the apparatus needed for our study a. )Petri Dish b. )Alcohol Lamp c. )Oven All of the enumerated materials above will be borrowed from the stockroom of Medical Laboratory Science Department. A letter of permission was given to the stockroom coordinator for authorized use of the materials stated above. Eosin Methylene Blue Agar (EMB) Procedure 1.Using a sterile cotton swab, swab on the surface of the peso bill then streak it onto an EMB agar. 2. Incubate plates at 35oC for twenty-four(24) hours. 3. After 24 hours, observe the plate as follows The colonies developing on EMB agar plates may be divides as Typical nucleated with or without metallic splendor Atypical opaque, pink mucoid, non-nucleated Negative no growth or clear watery, or clear colonies version of Data Philippine peso paper bill that will give a negative reaction on the EMB agar plate is considered not contaminated with E. coli and is safe for everyday domestic use.There will be no growt h or clear watery or clear colonies. On the other hand, water sample that yielded a positive result in E. coli analysis is considered to be contaminated with E. coli bacteria and is not safe. There would be a typical nucleated with or without metallic sheen. Central Philippine University Jaro, Iloilo City College of Arts and Sciences __________________ __________________ __________________ __________________ Sir/Madam, We, the 3rd yearBachelor in Medical Laboratory Science students of Central Philippine University who are soon enrolled in the subject Research is conducting a study entitled Prevalence of E. oli in Philippine Peso Bill. This is a requirement for the completion of the subject in Research 1. In line withthis, we are respectfully requesting you to be one of the respondents of our study by exchanging such peso bills 20, 50 and 100 respectively that has been collected as commuters fare. We need this data for the completion of this study. Your positive response to this req uest is highly appreciated. Thank you and God bless. Respectfully yours, JUSTINE GRACE JIMENEA ISAAC JAMES LEDESMA MAJA HANNAH LOU LOJA ROBERTO MANALO Jr. JIRAH PEDROSA Researchers Noted by DR. MA. 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